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mouse recombinant mouse (rm) il-3  (PeproTech)


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    Structured Review

    PeproTech mouse recombinant mouse (rm) il-3
    Mouse Recombinant Mouse (Rm) Il 3, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+recombinant+mouse+(rm)+il-3/interleukin+3/pm39496822-98-15-20
    Average 90 stars, based on 1 article reviews
    mouse recombinant mouse (rm) il-3 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: IL-10 promotes Th17 cell differentiation by enhancing STAT1-dependent IL-6 production via IgE-stimulated mast cells.
    Article Snippet: Briefly, bone marrow cells were cultured for 4–8 weeks in culture medium containing 20 ng/mL mouse recombinant mouse (rm) IL-3 (PeproTech, Rocky Hill, NJ, USA) at 37 °C in a humidified 5% CO2 incubator.

    Article Title: The development of murine bone marrow-derived mast cells expressing functional human MRGPRX2 for ex vivo and in vivo studies
    Article Snippet: Recombinant mouse interleukin-3 (IL-3) was obtained from Peprotech (Rocky Hill, NJ, USA).


    Article Title: p21/Zbtb18 repress the expression of cKit to regulate the self-renewal of hematopoietic stem cells
    Article Snippet: Recombinant murine SCF (250-03), recombinant murine TPO (315-14), recombinant murine IL-3 (213-13), and recombinant human EPO (100-64) were obtained from PeproTech.

    Article Title: IL-10 promotes Th17 cell differentiation by enhancing STAT1-dependent IL-6 production via IgE-stimulated mast cells
    Article Snippet: Briefly, bone marrow cells were cultured for 4–8 weeks in culture medium containing 20 ng/mL mouse recombinant mouse (rm) IL-3 (PeproTech, Rocky Hill, NJ, USA) at 37 °C in a humidified 5% CO 2 incubator.

    Article Title: A novel hyperactive variant of the Sleeping Beauty transposase facilitates non-viral genome engineering
    Article Snippet: Lin − cells isolated from the bone marrow of C57BL/6-Ly5.1 mice were cultured for 2 days in StemSpan SFEM Stem Span medium (STEMCELL Technologies, Vancouver, Canada) supplemented with 1% Pen/Strep (Thermo Fischer Scientific) and 10 ng/mL Recombinant Murine GM-CS, 25 ng/mL Recombinant Murine SCF, 25 ng/mL Recombinant Murine IL-3, 25 ng/mL Recombinant Murine IL-11, and 25 ng/mL Recombinant Murine TPO (all from PeproTech, Hamburg, Germany).

    Article Title: The role of mast cells and the underlying mechanisms in the formation of deep vein thrombosis in asthmatic mice.
    Article Snippet: Rationale: Asthma-induced inflammation has been shown to increase the risk of deep vein thrombosis (DVT), but the underlying mechanisms remain unclear.. Objective: To verify the associations between asthma exacerbation and the occurrence and development of DVT and explore the potential pathophysiological mechanisms at the molecular level.. Methods and results: By coculturing activated and unactivated mast cells with endothelial cells, we found that activated mast cells could lead to an increase in inflammatory factors.

    Article Title: WNK1 mediates M-CSF-induced macropinocytosis to enforce macrophage lineage fidelity
    Article Snippet: Resultant progenitor lineage-negative cells were collected, counted, and cultured in DMEM containing 20% heat-inactivated FBS, 50 ng/mL murine stem cell factor (PeproTech, 250-03), 10 ng/mL recombinant murine IL-3 (PeproTech, 213-13), and 20 ng/mL recombinant human IL-6 (PeproTech, 200-06) without PSQ for 48–72 h at 37 °C and 5% CO 2 (modified from ref. ).

    Recombinant:

    Article Title: IL-10 promotes Th17 cell differentiation by enhancing STAT1-dependent IL-6 production via IgE-stimulated mast cells.
    Article Snippet: Briefly, bone marrow cells were cultured for 4–8 weeks in culture medium containing 20 ng/mL mouse recombinant mouse (rm) IL-3 (PeproTech, Rocky Hill, NJ, USA) at 37 °C in a humidified 5% CO2 incubator.

    Article Title: The development of murine bone marrow-derived mast cells expressing functional human MRGPRX2 for ex vivo and in vivo studies
    Article Snippet: Recombinant mouse interleukin-3 (IL-3) was obtained from Peprotech (Rocky Hill, NJ, USA).


    Article Title: p21/Zbtb18 repress the expression of cKit to regulate the self-renewal of hematopoietic stem cells
    Article Snippet: Recombinant murine SCF (250-03), recombinant murine TPO (315-14), recombinant murine IL-3 (213-13), and recombinant human EPO (100-64) were obtained from PeproTech.

    Article Title: IL-10 promotes Th17 cell differentiation by enhancing STAT1-dependent IL-6 production via IgE-stimulated mast cells
    Article Snippet: Briefly, bone marrow cells were cultured for 4–8 weeks in culture medium containing 20 ng/mL mouse recombinant mouse (rm) IL-3 (PeproTech, Rocky Hill, NJ, USA) at 37 °C in a humidified 5% CO 2 incubator.

    Article Title: A novel hyperactive variant of the Sleeping Beauty transposase facilitates non-viral genome engineering
    Article Snippet: Lin − cells isolated from the bone marrow of C57BL/6-Ly5.1 mice were cultured for 2 days in StemSpan SFEM Stem Span medium (STEMCELL Technologies, Vancouver, Canada) supplemented with 1% Pen/Strep (Thermo Fischer Scientific) and 10 ng/mL Recombinant Murine GM-CS, 25 ng/mL Recombinant Murine SCF, 25 ng/mL Recombinant Murine IL-3, 25 ng/mL Recombinant Murine IL-11, and 25 ng/mL Recombinant Murine TPO (all from PeproTech, Hamburg, Germany).

    Article Title: The role of mast cells and the underlying mechanisms in the formation of deep vein thrombosis in asthmatic mice.
    Article Snippet: Rationale: Asthma-induced inflammation has been shown to increase the risk of deep vein thrombosis (DVT), but the underlying mechanisms remain unclear.. Objective: To verify the associations between asthma exacerbation and the occurrence and development of DVT and explore the potential pathophysiological mechanisms at the molecular level.. Methods and results: By coculturing activated and unactivated mast cells with endothelial cells, we found that activated mast cells could lead to an increase in inflammatory factors.

    Article Title: WNK1 mediates M-CSF-induced macropinocytosis to enforce macrophage lineage fidelity
    Article Snippet: Resultant progenitor lineage-negative cells were collected, counted, and cultured in DMEM containing 20% heat-inactivated FBS, 50 ng/mL murine stem cell factor (PeproTech, 250-03), 10 ng/mL recombinant murine IL-3 (PeproTech, 213-13), and 20 ng/mL recombinant human IL-6 (PeproTech, 200-06) without PSQ for 48–72 h at 37 °C and 5% CO 2 (modified from ref. ).

    Modification:

    Article Title: IL-10 promotes Th17 cell differentiation by enhancing STAT1-dependent IL-6 production via IgE-stimulated mast cells.
    Article Snippet: Briefly, bone marrow cells were cultured for 4–8 weeks in culture medium containing 20 ng/mL mouse recombinant mouse (rm) IL-3 (PeproTech, Rocky Hill, NJ, USA) at 37 °C in a humidified 5% CO2 incubator.

    Article Title: The development of murine bone marrow-derived mast cells expressing functional human MRGPRX2 for ex vivo and in vivo studies
    Article Snippet: Recombinant mouse interleukin-3 (IL-3) was obtained from Peprotech (Rocky Hill, NJ, USA).


    Article Title: p21/Zbtb18 repress the expression of cKit to regulate the self-renewal of hematopoietic stem cells
    Article Snippet: Recombinant murine SCF (250-03), recombinant murine TPO (315-14), recombinant murine IL-3 (213-13), and recombinant human EPO (100-64) were obtained from PeproTech.

    Article Title: IL-10 promotes Th17 cell differentiation by enhancing STAT1-dependent IL-6 production via IgE-stimulated mast cells
    Article Snippet: Briefly, bone marrow cells were cultured for 4–8 weeks in culture medium containing 20 ng/mL mouse recombinant mouse (rm) IL-3 (PeproTech, Rocky Hill, NJ, USA) at 37 °C in a humidified 5% CO 2 incubator.

    Article Title: A novel hyperactive variant of the Sleeping Beauty transposase facilitates non-viral genome engineering
    Article Snippet: Lin − cells isolated from the bone marrow of C57BL/6-Ly5.1 mice were cultured for 2 days in StemSpan SFEM Stem Span medium (STEMCELL Technologies, Vancouver, Canada) supplemented with 1% Pen/Strep (Thermo Fischer Scientific) and 10 ng/mL Recombinant Murine GM-CS, 25 ng/mL Recombinant Murine SCF, 25 ng/mL Recombinant Murine IL-3, 25 ng/mL Recombinant Murine IL-11, and 25 ng/mL Recombinant Murine TPO (all from PeproTech, Hamburg, Germany).

    Article Title: The role of mast cells and the underlying mechanisms in the formation of deep vein thrombosis in asthmatic mice.
    Article Snippet: Rationale: Asthma-induced inflammation has been shown to increase the risk of deep vein thrombosis (DVT), but the underlying mechanisms remain unclear.. Objective: To verify the associations between asthma exacerbation and the occurrence and development of DVT and explore the potential pathophysiological mechanisms at the molecular level.. Methods and results: By coculturing activated and unactivated mast cells with endothelial cells, we found that activated mast cells could lead to an increase in inflammatory factors.

    Article Title: WNK1 mediates M-CSF-induced macropinocytosis to enforce macrophage lineage fidelity
    Article Snippet: Resultant progenitor lineage-negative cells were collected, counted, and cultured in DMEM containing 20% heat-inactivated FBS, 50 ng/mL murine stem cell factor (PeproTech, 250-03), 10 ng/mL recombinant murine IL-3 (PeproTech, 213-13), and 20 ng/mL recombinant human IL-6 (PeproTech, 200-06) without PSQ for 48–72 h at 37 °C and 5% CO 2 (modified from ref. ).

    Isolation:

    Article Title: IL-10 promotes Th17 cell differentiation by enhancing STAT1-dependent IL-6 production via IgE-stimulated mast cells.
    Article Snippet: Briefly, bone marrow cells were cultured for 4–8 weeks in culture medium containing 20 ng/mL mouse recombinant mouse (rm) IL-3 (PeproTech, Rocky Hill, NJ, USA) at 37 °C in a humidified 5% CO2 incubator.

    Article Title: The development of murine bone marrow-derived mast cells expressing functional human MRGPRX2 for ex vivo and in vivo studies
    Article Snippet: Recombinant mouse interleukin-3 (IL-3) was obtained from Peprotech (Rocky Hill, NJ, USA).


    Article Title: p21/Zbtb18 repress the expression of cKit to regulate the self-renewal of hematopoietic stem cells
    Article Snippet: Recombinant murine SCF (250-03), recombinant murine TPO (315-14), recombinant murine IL-3 (213-13), and recombinant human EPO (100-64) were obtained from PeproTech.

    Article Title: IL-10 promotes Th17 cell differentiation by enhancing STAT1-dependent IL-6 production via IgE-stimulated mast cells
    Article Snippet: Briefly, bone marrow cells were cultured for 4–8 weeks in culture medium containing 20 ng/mL mouse recombinant mouse (rm) IL-3 (PeproTech, Rocky Hill, NJ, USA) at 37 °C in a humidified 5% CO 2 incubator.

    Article Title: A novel hyperactive variant of the Sleeping Beauty transposase facilitates non-viral genome engineering
    Article Snippet: Lin − cells isolated from the bone marrow of C57BL/6-Ly5.1 mice were cultured for 2 days in StemSpan SFEM Stem Span medium (STEMCELL Technologies, Vancouver, Canada) supplemented with 1% Pen/Strep (Thermo Fischer Scientific) and 10 ng/mL Recombinant Murine GM-CS, 25 ng/mL Recombinant Murine SCF, 25 ng/mL Recombinant Murine IL-3, 25 ng/mL Recombinant Murine IL-11, and 25 ng/mL Recombinant Murine TPO (all from PeproTech, Hamburg, Germany).

    Article Title: The role of mast cells and the underlying mechanisms in the formation of deep vein thrombosis in asthmatic mice.
    Article Snippet: Rationale: Asthma-induced inflammation has been shown to increase the risk of deep vein thrombosis (DVT), but the underlying mechanisms remain unclear.. Objective: To verify the associations between asthma exacerbation and the occurrence and development of DVT and explore the potential pathophysiological mechanisms at the molecular level.. Methods and results: By coculturing activated and unactivated mast cells with endothelial cells, we found that activated mast cells could lead to an increase in inflammatory factors.

    Article Title: WNK1 mediates M-CSF-induced macropinocytosis to enforce macrophage lineage fidelity
    Article Snippet: Resultant progenitor lineage-negative cells were collected, counted, and cultured in DMEM containing 20% heat-inactivated FBS, 50 ng/mL murine stem cell factor (PeproTech, 250-03), 10 ng/mL recombinant murine IL-3 (PeproTech, 213-13), and 20 ng/mL recombinant human IL-6 (PeproTech, 200-06) without PSQ for 48–72 h at 37 °C and 5% CO 2 (modified from ref. ).



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    Image Search Results


    SVPII and SVPIII enhanced proliferation of irradiated M-NFS-60 cells (applied alone or with IL-3). M-NFS-60 cells were irradiated for 5 min (5 Gy total dose) and then treated with PBS (control), 3 mg/L SVPII for 48 h (II), 3 mg/ml SVPIII for 48 h (III), 10 μg/L IL-3 for 48 h (IL-3), or the indicated combinations (II + IL-3, III + IL-3). * P < 0.01 vs. control; # P < 0.05 vs. II or vs. III).

    Journal: Cell & Bioscience

    Article Title: Scorpion venom peptide SPVII promotes irradiated cells proliferation and increases the expression of the IL-3 receptor

    doi: 10.1186/2045-3701-3-28

    Figure Lengend Snippet: SVPII and SVPIII enhanced proliferation of irradiated M-NFS-60 cells (applied alone or with IL-3). M-NFS-60 cells were irradiated for 5 min (5 Gy total dose) and then treated with PBS (control), 3 mg/L SVPII for 48 h (II), 3 mg/ml SVPIII for 48 h (III), 10 μg/L IL-3 for 48 h (IL-3), or the indicated combinations (II + IL-3, III + IL-3). * P < 0.01 vs. control; # P < 0.05 vs. II or vs. III).

    Article Snippet: Recombinant human macrophage colony stimulating factor (rhM-CSF) and recombinant mouse (rm) IL-3 were purchased from PeproTech Co. AlamarBlue™ was purchased from AbD Serotec (Great Britain), and membrane protein isolation kits were from Bio-Rad.

    Techniques: Irradiation

    Effect of SVPII on CFU count on 11th and 14th days in vitro (magnification: ×100). BM-MNCs (1 × 10 6 cells/mL) of BALB/C mouse were treated by: A: PBS (Control), B: cytokines (10 μg/L IL-3 and 62 μg/L rhM-CSF), C: 1 mg/L SVPII, D: 3 mg/L SVPII, E: 1 mg/L SVPII + cytokines, F: 3 mg/L SVPII + cytokines. Treated BM-MNCs were added into methyl cellulose half-solid medium and CFU was count on the 11th and 14th days.

    Journal: Cell & Bioscience

    Article Title: Scorpion venom peptide SPVII promotes irradiated cells proliferation and increases the expression of the IL-3 receptor

    doi: 10.1186/2045-3701-3-28

    Figure Lengend Snippet: Effect of SVPII on CFU count on 11th and 14th days in vitro (magnification: ×100). BM-MNCs (1 × 10 6 cells/mL) of BALB/C mouse were treated by: A: PBS (Control), B: cytokines (10 μg/L IL-3 and 62 μg/L rhM-CSF), C: 1 mg/L SVPII, D: 3 mg/L SVPII, E: 1 mg/L SVPII + cytokines, F: 3 mg/L SVPII + cytokines. Treated BM-MNCs were added into methyl cellulose half-solid medium and CFU was count on the 11th and 14th days.

    Article Snippet: Recombinant human macrophage colony stimulating factor (rhM-CSF) and recombinant mouse (rm) IL-3 were purchased from PeproTech Co. AlamarBlue™ was purchased from AbD Serotec (Great Britain), and membrane protein isolation kits were from Bio-Rad.

    Techniques: In Vitro

    Influence of SVPII on IL-3 receptor expression in M-NFS-60 cells (% IL-3R + cells). Control: cells treated with solvent (PBS); II: cells treated with 3 mg/L SVP II; IL-3: cells treated with 10 μg/L IL-3; II + IL-3: cells treated with 3 mg/L SVP II and 10 μg/L IL-3 simultaneously. * P < 0.05 vs. control group.

    Journal: Cell & Bioscience

    Article Title: Scorpion venom peptide SPVII promotes irradiated cells proliferation and increases the expression of the IL-3 receptor

    doi: 10.1186/2045-3701-3-28

    Figure Lengend Snippet: Influence of SVPII on IL-3 receptor expression in M-NFS-60 cells (% IL-3R + cells). Control: cells treated with solvent (PBS); II: cells treated with 3 mg/L SVP II; IL-3: cells treated with 10 μg/L IL-3; II + IL-3: cells treated with 3 mg/L SVP II and 10 μg/L IL-3 simultaneously. * P < 0.05 vs. control group.

    Article Snippet: Recombinant human macrophage colony stimulating factor (rhM-CSF) and recombinant mouse (rm) IL-3 were purchased from PeproTech Co. AlamarBlue™ was purchased from AbD Serotec (Great Britain), and membrane protein isolation kits were from Bio-Rad.

    Techniques: Expressing

    Immunofluorescence images demonstrating the influence of SVPII on IL-3 R expression by M-NFS-60 cells (×200). A. Control: cells treated with solvent (PBS); B. IL-3: cells treated with 10 μg/L IL-3; C. SVPII: cells treated with 3 mg/L SVP II; D. SVPII+IL-3: cells treated with 3 mg/L SVP II and 10 μg/L IL-3 simultaneously.

    Journal: Cell & Bioscience

    Article Title: Scorpion venom peptide SPVII promotes irradiated cells proliferation and increases the expression of the IL-3 receptor

    doi: 10.1186/2045-3701-3-28

    Figure Lengend Snippet: Immunofluorescence images demonstrating the influence of SVPII on IL-3 R expression by M-NFS-60 cells (×200). A. Control: cells treated with solvent (PBS); B. IL-3: cells treated with 10 μg/L IL-3; C. SVPII: cells treated with 3 mg/L SVP II; D. SVPII+IL-3: cells treated with 3 mg/L SVP II and 10 μg/L IL-3 simultaneously.

    Article Snippet: Recombinant human macrophage colony stimulating factor (rhM-CSF) and recombinant mouse (rm) IL-3 were purchased from PeproTech Co. AlamarBlue™ was purchased from AbD Serotec (Great Britain), and membrane protein isolation kits were from Bio-Rad.

    Techniques: Immunofluorescence, Expressing

    Influence of SVPII on IL-3R expression by M-NFS-60 cells. A. Normal culture condition: 10%FCM + rhM-CSF 62 μg/L); B. Low concentration of M-CSF: 10%FCM + rhM-CSF 15.5 μg/L; 1. Control: cells treated with solvent(PBS); 2. IL-3: cells treated with 10 μg/L IL-3; 3.SVPII: cells treated with 3 mg/L SVP II; 4. SVPII + IL-3: cells treated with 3 mg/L SVP II and 10 μg/L IL-3 simultaneously.

    Journal: Cell & Bioscience

    Article Title: Scorpion venom peptide SPVII promotes irradiated cells proliferation and increases the expression of the IL-3 receptor

    doi: 10.1186/2045-3701-3-28

    Figure Lengend Snippet: Influence of SVPII on IL-3R expression by M-NFS-60 cells. A. Normal culture condition: 10%FCM + rhM-CSF 62 μg/L); B. Low concentration of M-CSF: 10%FCM + rhM-CSF 15.5 μg/L; 1. Control: cells treated with solvent(PBS); 2. IL-3: cells treated with 10 μg/L IL-3; 3.SVPII: cells treated with 3 mg/L SVP II; 4. SVPII + IL-3: cells treated with 3 mg/L SVP II and 10 μg/L IL-3 simultaneously.

    Article Snippet: Recombinant human macrophage colony stimulating factor (rhM-CSF) and recombinant mouse (rm) IL-3 were purchased from PeproTech Co. AlamarBlue™ was purchased from AbD Serotec (Great Britain), and membrane protein isolation kits were from Bio-Rad.

    Techniques: Expressing, Concentration Assay

    Influence of SVPII on IL-3 R expression of M-NFS-60 cells after irradiation (% IL-3R + cells). After irradiated, the cells were treated with solvent(control) or 3 mg/L SVP II(II) or 10 μg/L IL-3(IL-3) or with 3 mg/L SVP II and 10 μg/L IL-3 simultaneously(II + IL-3). * P < 0.05 vs. control.

    Journal: Cell & Bioscience

    Article Title: Scorpion venom peptide SPVII promotes irradiated cells proliferation and increases the expression of the IL-3 receptor

    doi: 10.1186/2045-3701-3-28

    Figure Lengend Snippet: Influence of SVPII on IL-3 R expression of M-NFS-60 cells after irradiation (% IL-3R + cells). After irradiated, the cells were treated with solvent(control) or 3 mg/L SVP II(II) or 10 μg/L IL-3(IL-3) or with 3 mg/L SVP II and 10 μg/L IL-3 simultaneously(II + IL-3). * P < 0.05 vs. control.

    Article Snippet: Recombinant human macrophage colony stimulating factor (rhM-CSF) and recombinant mouse (rm) IL-3 were purchased from PeproTech Co. AlamarBlue™ was purchased from AbD Serotec (Great Britain), and membrane protein isolation kits were from Bio-Rad.

    Techniques: Expressing, Irradiation